Tumor/Normal pipeline


Databricks Runtime for Genomics is deprecated. Databricks is no longer building new Databricks Runtime for Genomics releases and will remove support for Databricks Runtime for Genomics on September 24, 2022, when Databricks Runtime for Genomics 7.3 LTS support ends. At that point Databricks Runtime for Genomics will no longer be available for selection when you create a cluster. For more information about the Databricks Runtime deprecation policy and schedule, see Supported Databricks runtime releases and support schedule. Bioinformatics libraries that were part of the runtime have been released as Docker Containers, which you can find on the ProjectGlow Dockerhub page.

The Databricks tumor/normal pipeline is a GATK best practices compliant pipeline for short read alignment and somatic variant calling using the MuTect2 variant caller.


The pipeline consists of the following steps:

  1. Normal sample alignment using BWA-MEM.

  2. Tumor sample alignment using BWA-MEM.

  3. Variant calling with MuTect2.


The pipeline is run as a Databricks job. You can set up a cluster policy to save the configuration:

  "num_workers": {
    "type": "unlimited",
    "defaultValue": 13
  "node_type_id": {
    "type": "unlimited",
    "defaultValue": "c5.9xlarge"
  "spark_env_vars.refGenomeId": {
    "type": "unlimited",
    "defaultValue": "grch38"
  "spark_version": {
    "type": "regex",
    "pattern": ".*-hls.*",
    "defaultValue": "7.4.x-hls-scala2.12"
  "aws_attributes.ebs_volume_count": {
    "type": "unlimited",
    "defaultValue": 3
  "aws_attributes.ebs_volume_size": {
    "type": "unlimited",
    "defaultValue": 200
  • The cluster configuration should use Databricks Runtime for Genomics.

  • The task should be the tumor/normal notebook found at the bottom of this page.

  • For best performance, use compute optimized instances with at least 60GB of memory. We recommend c5.9xlarge.

  • If you’re running base quality score recalibration, use general purpose (m5.4xlarge) instances instead since this operation requires more memory.

  • To reduce costs, use all spot workers with the Spot fall back to On-demand option selected.

  • Attach 3 200GB SSD EBS volumes

Reference genomes

You must configure the reference genome using an environment variable. To use GRCh37, set the environment variable:


To use GRCh38, change grch37 to grch38.

To use a custom reference genome, see instructions in Custom reference genomes.


The pipeline accepts parameters that control its behavior. The most important and commonly changed parameters are documented here. To view all available parameters and their usage information, run the first cell of the pipeline notebook. New parameters are added regularly. After importing the notebook and setting it as a job task, you can set these parameters for all runs or per-run.






The manifest describing the input.



The path where pipeline output should be written.



  • If skip, stages will be skipped if output already exists.

  • If overwrite, existing output will be deleted.



If true, the pipeline writes results to a VCF file as well as Delta.



A timeout applied per sample. After reaching this timeout, the pipeline continues on to the next sample. The value of this parameter must include a timeout unit: ‘s’ for seconds, ‘m’ for minutes, or ‘h’ for hours. For example, ‘60m’ results in a timeout of 60 minutes.


To optimize run time, set the spark.sql.shuffle.partitions Spark configuration to three times the number of cores of the cluster.

Manifest format


Manifest blobs are supported in Databricks Runtime 6.6 for Genomics and above.

The manifest is a CSV file or blob describing where to find the input FASTQ or BAM files. For example:


If your input consists of unaligned BAM files, you should omit the paired_end field:


The tumor and normal samples for a given individual are grouped by the pair_id field. The tumor and normal sample names read group names must be different within a pair.


If the provided manifest is a file, the file_path field in each row may be an absolute path or a path relative to the manifest file. If the provided manifest is a blob, the file_path field must be an absolute path. You can include globs (*) to match many files.

Additional usage info and troubleshooting

The tumor/normal pipeline shares many operational details with the other Databricks pipelines. For more detailed usage information, such as output format structure, tips for running programmatically, steps for setting up custom reference genomes, and common issues, see DNASeq pipeline.


The pipeline was renamed from TNSeq to MutSeq in Databricks Runtime 7.3 LTS for Genomics and above.

MutSeq pipeline notebook

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TNSeq pipeline notebook (Legacy)

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